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Western blot imaging system is a unique technique in the life science lab where the proteins are separated based on their molecular weight using gel electrophoresis. Chemiluminescence is the most popular western blot detection method due to its superior level of sensitivity, and imaging systems pitched towards the use along with the technique are available to additionally optimize the pace, stability of the signal, sensitivity, and user-friendliness. During the process at the end of electrophoresis, the estranged molecules are transferred to a second matrix which comprises of nitrocellulose membrane or polyvinylidene difluoride (PVDF). To avoid any kind of nonspecific adhering of protein antibodies to the surface of the membrane, it is arrested with a particular mechanism. There are two kinds of western blotting techniques – direct detection and indirect detection methods. The direct method possesses qualities such as swift effect owing to the presence of one antibody and primary antibodies. However, the disadvantage associated with this method is that it consists of lessened immunoreactivity of key antibody termed as primary antibody, low flexibility, non cost-efficient. Additionally, the indirect variant has advantages such as potential options of labeled secondary antibodies, secondary antibody amplifying signal, and primary antibody immunoreactivity stays unaltered by labeling.
The global western blot imagers industry was valued at US $510.1 million in 2014 and it garnered more than 25% of the global molecular diagnostics market. By the end of 2021, this industry’s market is projected to probably reach US $725.0 million, while growing at a CAGR of 5.4% during the forecast period owing to the increased occurrence of HIV and constant research in proteomics segment. More percentage of expenditure and increasing investment in the R&D by several biotechnology and pharmaceutical companies are two key factors contributing towards the lucrative growth of the western blot imagers market. The outgrowth of substitute method for the detection of protein and a superior price of products will probably hinder the market growth of global western blot imagers industry. A few prime trends in this industry include implementation of the automated enhanced techniques, innovation of latest and new imaging software for analysis of western blot results, and steady investment in R&D and an upsurge in acquisitions and mergers.