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In Brief: A Breakthrough in Monocot Transformation Methods – Plant Science Today

Monday, September 12, 2016 18:45

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At Plant Science Today

This week’s Research in Focus is reprinted from an In Brief published by Science Editor Nancy Hofmann in The Plant Cell, which summarizes an article newly published by Lowe et al. This study explores the dual and opposing roles of the enzyme xanthine dehydrogenase in modulation of ROS metabolism during defense responses in Arabidopsis.

The ability to generate transgenic plants without regard to cultivar or genotype can be considered a holy grail of cereal crop transformation. Despite years of effort, it has been remarkably difficult to develop efficient methods for transformation of cereals. The preferred methods generally involve Agrobacterium-mediated transformation of cultured tissue or immature embryos, followed by callus culture to regenerate plants (reviewed inShrawat and Lörz, 2006). Unfortunately, the capacity of Agrobacterium to infect monocots is limited to a narrow range of genotypes and the utility of the technique is further limited by the recalcitrance of many genotypes to callus formation and regeneration. ABreakthrough Report from Lowe et al. (2016) describes an exciting new approach to boost monocot transformation rates in a broad range of genotypes….

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Source: http://gmopundit.blogspot.com/2016/09/in-brief-breakthrough-in-monocot.html