CALIFORNIA STATE SCIENCE FAIR: Will Colloidal Silver Work as an Antibacterial?

Wednesday, October 3, 2012 11:50

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CALIFORNIA STATE SCIENCE FAIR

2009 PROJECT SUMMARY

Ap2/09

Name(s) Project Number

Will Colloidal Silver Work as an Antibacterial?

Objectives/Goals

The objective is to find out if colloidal silver would eliminate the bacteria colonies of Escherichia coli,

Pseudomonas aeruginosa and Staphylococcus aureus.

Methods/Materials

In the initial colony tube, the organism was diluted to 0.5 McFarland standard using the turbidity meter. I

used Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa

ATCC 27853. The initial colony tube was diluted to 100μ added to 25ml of silver solution to subculture

plates (BAP) at 0 hrs. of incubation, at 2 hrs. of incubation (at 36.8° 6%CO²) and at 24 hrs (at 36.8°

6%CO²). The amount of fluid used to inoculate plates was 100μ of organisms to the center of the plate,

streaked in three different directions across the plates. I ended up with three plates used as my “Control”

and three plates as my “Variable.” Results were read at 0 hrs (full fields), 24 hrs and 48 hrs.

Results

The results of this experiment proved that colloidal silver has properties that disable the production and

terminates the growth of bacteria. Escherichia coli colonies were killed after 2 hours of incubation, 24

hours readings. Pseudomonas aeruginosa and Staphylococcus aureus colonies were completely killed after

24 hours of incubation, 48 hours readings.

Conclusions/Discussion

This project had an exciting outcome since colloidal silver is used only as a dietary supplement. In the

future more research can be done so that it can be used as an antibiotic to kill fungi, viral and bacterial

diseases. In addition to the benefits of colloidal silver, the cost would be very minimal since it could be

made at home.

This project proves that colloidal silver, used as a dietary supplement, is an effective antibacterial to kill

Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli.

Used lab equipment at El Centro Regional Medical Hospital under the supervision of Magda Rumbout-

Clinical Lab Scientist.

NutraSilver has coinducted the same lab test, except we used FDA-certified labs. We got the same results.

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