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I was hoping to get suggestions from the readers of AA about methods of tissue collection for genetic work other than tail tips. I’ve been working with the agamid lizard Sitana ponticeriana, and my work is now taking decidedly genetic directions. It remains unclear whether or not these lizards regenerate their lost tails–while they seem to lose tails easily, I didn’t see any lizards with noticeably regenerated tails in the field. Given this, I am a little uncomfortable with the idea of taking tail tips as tissue for genetic work. Are there other common and easy options for sampling tissue from lizards? Many thanks in advance for your responses!
(Feel free also to weigh in with whether or not you think it acceptable to collect tail tips in a species that certainly autotomizes its tail but does not grow it back–it seems like a grey area to me).
2013-01-16 07:20:54
Source: http://www.anoleannals.org/2013/01/16/tissue-for-genetic-material-options-other-than-tail-tips/